| Atlanta United FC | Atlanta | 2014 | Mercedes-Benz Stadium, Bobby Dodd Stadium |
| Austin FC | Austin | 2018 | Q2 Stadium |
| CF Montréal | Montreal | 2010 | Saputo Stadium |
| Charlotte FC | Charlotte | December 17, 2019 | Bank of America Stadium |
| Chicago Fire FC | Chicago | October 8, 1997 | Soldier Field, Benedetti–Wehrli Stadium, SeatGeek Stadium |
| Colorado Rapids | Commerce City | 1995 | Dick's Sporting Goods Park |
| Columbus Crew | Columbus | 1994 | Ohio Stadium, Historic Crew Stadium, ScottsMiracle-Gro Field |
| D.C. United | Washington, D.C. | 1994 | Audi Field, Robert F. Kennedy Memorial Stadium |
| FC Cincinnati | Cincinnati | January 27, 2017 | Nippert Stadium, TQL Stadium |
| FC Dallas | Frisco | 1995 | Toyota Stadium |
| Houston Dynamo FC | Houston | 2005 | Shell Energy Stadium |
| Inter Miami CF | Miami | January 29, 2018 | Chase Stadium |
| LA Galaxy | Los Angeles | 1995 | Dignity Health Sports Park |
| Los Angeles FC | Los Angeles | 2014 | BMO Stadium |
| Minnesota United FC | Minneapolis | 2015 | Allianz Field, Huntington Bank Stadium |
| Nashville SC | Nashville | 2017 | Geodis Park, Nissan Stadium |
| New England Revolution | Foxborough | 1995 | Gillette Stadium |
| Orlando City SC | Orlando | 2013 | Inter&Co Stadium, Camping World Stadium |
| Philadelphia Union | Chester | 2008 | Subaru Park |
| Portland Timbers | Portland | 2009 | Providence Park |
| Real Salt Lake | Salt Lake City | 2005 | America First Field |
| Red Bull New York | Harrison | 1995 | Giants Stadium, Sports Illustrated Stadium |
| San Diego FC | San Diego | May 18, 2023 | Snapdragon Stadium |
| San Jose Earthquakes | San Jose | 1995 | PayPal Park, Stevens Stadium |
| Seattle Sounders FC | Seattle | 2009 | Lumen Field |
| Sporting Kansas City | Kansas City | 1995 | Sporting Park, Legends Field, Arrowhead Stadium |
| St. Louis City SC | St. Louis | August 20, 2019 | Energizer Park |
| Toronto FC | Toronto | 2006 | BMO Field |
| Vancouver Whitecaps FC | Vancouver | 2009 | BC Place, Empire Field |